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Histology

The Core Facility Histology operates all necessary equipment for the traditional preparation of histological samples at the Center for Medical Research, for processing at room temperature (FFPE samples) as well as at low temperatures (cryofixation). It is equipped with modern imaging techniques and scanners that enable rapid analysis in brightfield and fluorescence, as well as the identification of over 100 biological markers in a single tissue section. In addition to training on equipment, the Core Facility Histology also offers consultations in the areas of sample preparation, sample processing, and image analysis.

 

Devices

The HistoCore Arcadia H is a modern paraffin embedding station from Leica. It is used for embedding histological tissue samples, after fixation, into liquid paraffin.  Before solidification into paraffin the tissue is properly oriented with tweezers.

Leica HistoCore Arcadia H, opens a file in a new window

The RM2245 from Leica is a semi-automatic rotary microtome used for cutting formalin-fixed, paraffin-embedded histological tissues, particularly for users who prefer manual cutting.

Leica RM2245, opens a file in a new window

The HistoCore AUTOCUT is a motorized rotary microtome that can be used both manually and fully motorized. This device is capable of cutting formalin-fixed paraffin-embedded tissues of various hardness grades at different section thicknesses.

Leica HistoCore AUTOCUT R, opens a file in a new window

The Leica CM3050 S is a semi-motorized high-performance cryostat with the option for motorized cutting. It is used to rapidly freeze fresh histological samples and cut them while in a frozen state.

Leica CM3050 S, opens a file in a new window

The Leica VT1200 S is primarily used to cut fresh biological tissue in a cooled buffer. The goal is to preserve the integrity of the tissue cells for further biological processes. Cutting with the VT1200 S is motorized.

Leica VT1200 S, opens a file in a new window

The PhenoCycler-Fusion System by Akoya BioSciences enables spatial ultra-high-plex phenotyping of entire slides with single-cell resolution through automated fluidics and iterative imaging. The automated cycling and image capture are facilitated by the integration of flow cells placed in a slide holder. This allows the detection of over 100 markers on a single intact tissue. This device is operated by the Core Facility and is not independently operated by users.

PhenoCycler-Fusion System Akoya, opens a file in a new window

Services

  • Phenotyping (PhenoCycler-Fusion System)
  • Equipment and Equipment Training:
    • Histological Sample Processing
    • Tissue sectioning in Paraffin and Fresh Frozen
    • Microwave Tissue Processing
    • Slide Scanning in Brightfield and Fluorescence
  • Troubleshooting
  • Consultation on Project Planning
  • Consultation on Sample Processing
Claudia Himmelbauer beim Arbeiten am Akoya

Contact

Name

Position

Phone

E-mail Address

Claudia Himmelbauer, MSc

Head of Core Facility

0732 2468  3093
0664 602468213

claudia.himmelbauer@jku.at

Gewebefotos PhenoCyler-Fusion