It is our aim to obtain a detailed understanding of ion channel mechanisms at the structural level in the living cell which is crucial for the development of future therapeutic approaches. To achieve this goal, we use a combined approach of the novel genetic code expansion technology together with a set of traditional methods including molecular biology, biochemistry, Ca²⁺ imaging, electrophysiology and fluorescence microscopy. Specifically, we

• Characterize biophysical characteristics of ion channels.

• Study interactions of ion channels, both within their complex and with their regulatory proteins.

• Investigate the pharmacology of ion channels.

• Transfer novel biophysical and biochemical properties (e.g. light-sensitivity) to single amino acids of ion channels.